Place an elodea leaf in the drop of water, place a coverslip on top. Dna extraction from cheek cells woodland hills school. Add a small drop of iodine stain directly to the tissue. It was only after microscopes were developed and we were able to view the. Introduction to cells, cheek cell and onion cell lab. Cheek cells the cheek cells below were both viewed with a brightfield microscope at 400x total magnification. In the meantime, this lab will further demystify dna by allowing you to see it for your own eyes as a rather abundant substance found in virtually all of your bodys cells. The light microscope used in this lab is not powerful enough to view other organelles in the cheek cell. Holding your test tube at an angle, carefully add the 2ml of cell lysis solution to your test tube that should already contain your collected cheek cells. The study of cell structure and function is known as cytology. Make a drawing of one onion cell, labeling all of its parts as you observe them. This activity provides an opportunity to accomplish two tasks in our ap biology adventure. Observe under low power first 4x, then under high power 10x draw in figure 9.
However, you can observe the cell wall, nucleus, and cytoplasm. Stir the end of the toothpick into the stain and throw the toothpick away. Nucleus, cell wall, cytoplasm, and the nucleolus within the cells of an onion and a cheek. Onion tissue provides excellent cells to study under the microscope. To observe and identify major animal cell structures and to relate the structure of the cell to its function. Iodine and methylene blue are required for this experiment and are common in any schools science lab department. Although there are many differences between types of cells, all cells have similar structure and function.
Students will discover that onions are made up of cells. The clip is small and inexpensive and it seems to work well and theres even included software with it to track many more details or. Gently scrape the inside of your cheek with the flat side of a toothpick. Put a drop of water in the center of a glass slide. The purpose of this lab is to learn how to prepare a wet mound, to learn proper staining techniques and to examine human cheek cells and onion skin cells. First of all, we will hopefully be able to see our own dna from. Place a small amount of onion tissue on the glass slide. Compare and contrast prokaryotic and eukaryotic cells, 2. Cheek cells lab analysis 3 1 cheek cells the cheek cells. Holding your test tube at an angle, carefully add the 2ml of cell lysis solution to your test tube that.
Lab schedule each week a quiz will be given at the beginning of each lab covering the material presented from the previous lab. Introductionafter reading through the lab procedures in parts a and b, write a thorough introductionexplaining the purpose of this lab. Dna extraction from cheek cells the same general procedures, with little modification, for extracting dna can be used with a variety of plant and animal cells. A neat lab sheet introduces cell biologists to the general structure of both animal and plant cells. Identify each cell part and state its function, and. The light microscope used in the lab is not powerful enough to view other organelles in the cheek cell.
Rotate the nosepiece back to low power and remove the slide. Chemical reactions involving the transfer of electrons from one reactant to another are called. Dna extraction lab 1 cheek cell dna extraction capture your genes in a bottle. Deoxyribonucleic acid dna is a molecule present in all living things, including bacteria, plants, and animals. First week safety contracts, syllabus overview week 2. In this lab, you will prepare and observe cells from both a plant onion and an animal your own cheek. Obtain a small amount of cell lysis solution from the front counter using your small beaker. Red blood cells stain redorange, nuclei stain bluepurple and cytoplasm stains blue to pink.
Human cheek cell worksheet for 8th 10th grade lesson. Every time a cell divides for growth, repair, or reproduction the dna within the cells nucleus is copied and then coiled tightly into chromosomes. Observe the onion cell under both low and high power. Cells continually exchange materials with their external environments. You will need to provide slides of cheek cells, onion cells, elodea, and volvox. Cell studies the cell theory states that all living organisms are made of cells. Osmosis is the process whereby water moves across a cell membrane by diffusion. This laboratory course is designed to familiarize you with techniques used in. Keeping this in mind, what organelle do you think would be numerous inside the cells of your mouth.
Cheek cell dna extraction capture your genes in a bottle. List 2 organelles that were not visible but should have been in the cheek cell. They draw and label what they observe under high power. It was only after microscopes were developed and we were able to view the universality of cells that this theory was accepted.
Draw a three cell grouping and label at least 2 cell structures that you can see. For example, you will observe a large circular nucleus in each cell, which contains the genetic material for the cell. Dna carries genetic information that is inherited, or passed down from parents to offspring. Get up close and personal with human cells with this lab worksheet. Label the cell membrane, nucleus, cytoplasm, and any other organelles youobserve i. It is predicted that all cells, no matter if it be plant or animal, will be in beside each other in large groups, that most of the major organelles will be visible through the microscope at. Draw your observations on your paper for low, medium, and high powers. View lab report cheek cells from bio 206l at university of texas. The nucleus is present at the periphery of the cytoplasm. Please follow all rules of a true biological drawing.
Dna extraction from cheek cells home woodland hills. Many of the organelles cannot be seem with a light microscope because they are generally clear or too small. Nk cell cytotoxic activity was determined by the number of apoptotic and lysed k562 cells. If you have difficulty printing out this page, try the pdf version. An onion is a multicellular consisting of many cells plant organism. As in all plant cells, the cell of an onion peel consists of a cell wall, cell membrane, cytoplasm, nucleus and a large vacuole. In class, we obtained cheek cells by scraping the inside of the mouth with a toothpick and then rubbing the toothpick on a drop of water with blue. Students will observe cheek cells under a microscope. How was the onion cell different from the cheek cell. Using a toothpick, scrape a tiny amount of tomato pulp immediately. Human cheek cell worksheet for 8th 10th grade lesson planet. This differentiation of cells is the result of expressing different genes in different amounts, however all genes are present in all cells. The cells of an onion skin are generally rectangular in shape and range in size from 0.
These neat drawings must be completed using a pencil. With only a few exceptions, dna is found within practically every cell of an organisms body. At your lab station, measure 2 ml of cell lysis solution into a graduated cylinder. Although cells are the building block of all living organisms, different types of organisms have. The openlab is an opensource, digital platform designed to support teaching and learning at city tech new york city college of technology, and to promote student and faculty engagement in the intellectual and social life of the college community. Learners use a microscope to examine their own cheek cells, drawing diagrams of the cells and identifying the parts when they have focused in on a visible specimen. The exchange of such materials is controlled by a plasma membrane composed of a.
Review of cellab clip fitness activity tracker technogog. Students can easily view their own cells as an example of eukaryote cells and cell theory. Create a lab report beginning on page 70 of your inb. If you missed the microscope lab we did in class, you will need to make it up by using a virtual microscope which can be accessed on the internet.
Onion skin cells have many rectangular cells that are easy to see under a light microscope. The cell itself and again the nucleus within the cell are both surrounded by membranes made primarily of phospholipids. The vacuole is prominent and present at the centre of the cell. Students will discover that their skin is made up of cells. Introduction to cells, cheek cell and onion cell lab, lesson powerpoint 19,369 views. In this lab activity you will observe the effects of osmosis on plant cells. Write a short description of each of the following. The following labeled drawings should be completed on your own paper. In our cells, a compartment of the cell called the nucleus contains the dna. Students will observe onion cells under a microscope. Cell structure and function cells are the basic unit and structure of all living organism. In this lab students will be able to observe the following organelles. If you missed the lab we did in class, you will need to make it up by using a virtual microscope which can be.
At minimum you should observe the cell membrane, nucleus, and cytoplasm. Draw a diagram of one cheek cell and label its parts. Introduction to cells, cheek cell and onion cell lab, lesson. For example, you will observe a large circular nucleus in each. Aug 06, 20 introduction to cells, cheek cell and onion cell lab, lesson powerpoint. Today%for%this%laboratory%we%will%be%using%a%compound%light%microscope. This one period lab is a great way to introduce microscope skills or view animal cells using the students own cheek cells. List 2 organelles that were not visible but could be found in cells if you had a microscope with a better magnification.
This human cheek cell worksheet is suitable for 8th 10th grade. Most commercial laboratories use some form of romanowskytype. Observe under the microscope under low, medium and high powers. The main cell structures are easy to see when viewed with the microscope at low power.
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